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NGF and its precursor pro-NGF in human peritubular cells and culture supernatant. A, immunocytochemistry of NGF and pro-NGF in cultured cells (n = 2). B, NGF (17 kDa) and pro-NGF (37 kDa) were detected in lysates of HTPC/-Fs (L; n = 12) and in the conditioned culture medium (S; n = 6) by Western blotting. Note that the NGF antibody recognizes both pro-NGF and NGF, whereas the pro-NGF antibody does not recognize NGF.
