FIGURE 5.

Tryptase can cleave pro-NGF. A, a reduction in the levels of pro-NGF became obvious by Western blot detection of pro-NGF (n = 3) after treatment of the supernatant of HTPCs with or without 1000 ng/ml tryptase for 24 h. B, Western blot studies indicate a decrease of synthetic pro-NGF after treatment with tryptase (1000 ng/ml; 24 h; n = 8) but no decrease by SLIGKV (100 μm; n = 8) or heat-inactivated tryptase (1000 ng/ml; n = 7). Specificity of the pro-NGF antibody was shown by preadsorption (n = 2).