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. 2011 Jul 18;286(36):31707–31713. doi: 10.1074/jbc.M111.233486

FIGURE 6.

FIGURE 6.

Tryptase cleaves pro-NGF in vitro. A, cleavage sites of tryptase in the pro-NGF sequence (blue) and mature NGF sequence (black) as found by LC-MS/MS analyses in vitro using synthetic human pro-NGF and recombinant human tryptase. Arrows indicate cleavage sites. The cleavage site that is shared by tryptase, furin, and proconvertases is indicated by a red arrow. Note the multiple cleavage sites of the enzyme tryptase within the pro-NGF molecule and the three sites within the mature NGF region. B, MS/MS spectrum of peptide SSSHPIFHR. The N terminus of the peptide represents the N terminus of functional NGF. Annotation of characteristic y and b fragment ions was performed using Scaffold 2.04 software. C, MS/MS spectrum of peptide ALTMDGKQAAWR. The peptide represents an internal fragment of functional NGF. Annotation of characteristic y and b fragment ions was performed using Scaffold 2.04 software.