TABLE 1.

MS/MS analysis of recombinant pro-NGF treated with tryptase

Shown is a list of peptides identified with precursor mass accuracies <5 ppm and Mascot ion scores >25. Arrows indicate tryptase cleavage sites. Start and stop positions refer to the sequence of the recombinant pro-NGF. M(ox), oxidized methionine.

	Ion score	Delta mass	Start	Stop
		ppm
-↓MEPHSESNVPA↓G	30	1.73	1	11
-↓MEPHSESNVPAG↓H	32	0.46	1	12
-↓MEPHSESNVPAGH↓T	61	1.21	1	13
-↓M(ox)EPHSESNVPAGH↓T	65	1.25	1	13
H↓TIPQVHWTK↓L	27	−0.73	14	22
K↓LQHSLDT↓A	50	1.59	23	29
K↓LQHSLDTALR↓R	60	−0.01	23	32
K↓LQHSLDTALRR↓A	28	0.46	23	33
Q↓HSLDTALR↓R	36	−0.35	25	32
H↓SLDTALR↓R	49	0.74	26	32
L↓DTALR↓R	34	0.26	28	32
R↓ARSAPAAAIAAR↓V	48	0.76	34	45
A↓RSAPAAAIAAR↓V	57	0.91	35	45
R↓SAPAAAIAAR↓V	85	0.07	36	45
S↓APAAAIAAR↓V	48	−1.16	37	45
A↓PAAAIAAR↓V	61	1.57	38	45
A↓AIAAR↓V	28	1.29	41	45
T↓RNITVDPR↓L	31	1.13	51	58
R↓NITVDPR↓L	40	0.83	52	58
R↓NITVDPRLFK↓K	41	0.97	52	61
N↓ITVDPR↓L	29	0.82	53	58
R↓SPRVLF↓S	27	1.21	67	72
P↓RVLFSTQPPR↓E	35	2.22	69	78
R↓VLFSTQPPR↓E	72	−1.17	70	78
F↓EVGGAAPFNR↓T	37	0.26	89	98
R↓SSSHPIFHR↓G	28	0.45	105	113
K↓ALTMDGKQAAWR↓F	50	2.66	192	203