FIGURE 5.
The dependence of k0 with 1/T allows calculation of the unfolding energy barrier and the collision frequency factor. From the Arrhenius/Bell fit shown in Fig. 3, we obtained the value of the unfolding rate in the absence of force k0 for each temperature probed: 5 °C (triangle), 15 °C (inverted triangle), 25 °C (circle), 35 °C (diamond), and 45 °C (square). The solid black line represents the fit of Equation 1 to the data, giving rise to values for the activation barrier of ΔG‡ = 71 ± 5 kJ/mol and for exponential prefactor, υ‡ = ∼109.6 ± 0.99 (±S.D.), corresponding to a mean value of υ‡ = 4 × 109 s−1.