FIGURE 5.

The structure of PenP-E166Cb with cephaloridine acylated at its active site. a, the fo-fc density map of cephaloridine in the complex structure of PenP-E166Cb-cephaloridine is shown in mesh format in gray and contoured at 2.0 σ. The disordered BADAN molecule is illustrated in dashed lines. The yellow dotted line represents the poorly ordered segment of residues L169 to V174 on the Ω-loop. b, overlapping of PenP-E166Cb (lemon) and PC1 (coral) with cephaloridine. PenP-E166Cb and PC-1 are shown in green and red, respectively. Residues S70, N104, and cephaloridine are displayed in a cpk cylinder model. The blue dotted line represents the H-bond between side chains of N104 and acylamide of cephaloridine.