FIGURE 2.

Co-immunoprecipitation of GlcAT-P and β4GalT-II in N2a cells. Lysate of N2a cells transiently expressing FLAG-GlcAT-P, β4GalT-I-myc, or β4GalT-II-myc was immunoprecipitated (IP) with anti-FLAG pAb (A, IP: FLAG) or anti-Myc pAb (B, IP: myc), subjected to SDS-PAGE, and Western-blotted (Blot) with anti-FLAG mAb or anti-Myc mAb. To examine the level of each protein, the cell lysates were directly subjected to SDS-PAGE and Western blotting with anti-FLAG mAb or anti-Myc mAb (input).