FIGURE 3.

ER retention assays using GlcAT-P-AAA. A, schematic diagrams of GlcAT-P and GlcAT-P-AAA. CT, cytoplasmic tail, TM, transmembrane domain. B–M, N2a cells were transiently co-transfected with GlcAT-P or GlcAT-P-AAA and β4GalT-I-myc or β4GalT-II-myc. GlcAT-P and GlcAT-P-AAA were detected with GP2 pAb (B, E, H, and K) and Alexa 546-conjugated secondary antibodies. β4GalT-myc (I and II) was detected with anti-Myc mAb (C, F, I, and L) and Alexa 488-conjugated secondary antibodies. D, G, J, and M, overlaid images. Bar, 10 μm.