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Immunoprecipitation using PST-FLAG and β4GalT-myc in N2a cells. Lysate of N2a cells transiently expressing PST-FLAG, β4GalT-I-myc, or β4GalT-II-myc were immunoprecipitated (IP) with anti-FLAG pAb (IP: FLAG), subjected to SDS-PAGE, and Western-blotted with anti-FLAG mAb or anti-Myc mAb. To examine the level of each protein, the cell lysates were directly subjected to SDS-PAGE and then Western blotting (WB) with anti-FLAG mAb or anti-Myc mAb (input).
