TABLE 1.
Changes in cytoplasmic pH and membrane potential from oocytes expressing PvAMT1;1 or the mutant H211E and exposed to 1 mm NH4+
Results correspond to acidification of cytoplasmic pH (ΔpH) and depolarization of membrane potential (ΔMP). Negative values for pH changes represent acidification of the cytoplasm in order to correspond to a decrease in pH. Depolarization of the membrane potential corresponds to positive values. Data are the mean ± S.D. from at least four oocytes.
| ΔpH |
ΔMP |
|||
|---|---|---|---|---|
| pH 7.0 | pH 5.5 | pH 7.0 | pH 5.5 | |
| pH units | mV | |||
| PvAMT1;1 | −0.199 ± 0.018 | −0.125 ± 0.035 | 30.9 ± 10.9 | 52.1 ± 15.5 |
| H2O | −0.043 ± 0.016 | 0.043 ± 0.028 | 2.6 ± 2.9 | −1.1 ± 1.5 |
| H211E | −0.050 ± 0.026 | −0.022 ± 0.016 | 27.4 ± 8.0 | 43.9 ± 1.3 |