TABLE 2.
Activity of the PvAMT1;1 His125 mutants
Values correspond to the currents activated by the various cations at 1 mm at a holding potential of −140 mV. Values are the mean ± S.D. of no less than 10 oocytes. MetAm corresponds to methylammonium.
| NaCl |
KCl |
MetAm |
Ammonium |
|||||
|---|---|---|---|---|---|---|---|---|
| pH 7.0 | pH 5.5 | pH 7.0 | pH 5.5 | pH 7.0 | pH 5.5 | pH 7.0 | pH 5.5 | |
| I (nA) | ||||||||
| H2O | 5.5 ± 2.0 | 4.7 ± 1.9 | 18.2 ± 6.8 | 5.9 ± 2.3 | 7.2 ± 2.6 | 3.1 ± 1.6 | 7.3 ± 2.0 | 2.6 ± 1.8 |
| PvAMT1;1 | 8.1 ± 3.3 | 10.1 ± 2.8 | 8.7 ± 3.9 | 5.9 ± 2.3 | 20.3 ± 6.9 | 51.2 ± 13.3 | 36.4 ± 8.2 | 78.9 ± 9.9 |
| H125R | 7.1 ± 1.4 | 7.1 ± 1.4 | 3.7 ± 2.1 | 5.2 ± 1.0 | 51.0 ± 15.2 | 74.5 ± 17.5 | 103.1 ± 31.7 | 176.8 ± 35.1 |
| H125A | 5.6 ± 0.7 | 4.6 ± 2.2 | 4.3 ± 3.1 | 1.8 ± 1.2 | 5.1 ± 2.0 | 11.7 ± 6.7 | 0.3 ± 3.0 | 20.4 ± 8.0 |