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. 2011 Jul 11;286(36):31064–31071. doi: 10.1074/jbc.M111.235457

FIGURE 5.

FIGURE 5.

PI3K mediates estrogen activated hERα transcriptional activation of hSlo1. A and B, activity of the 2.5-kb hSlo1 promoter in control (no hERα) and DBDM-hERα in the absence (vehicle, dark gray bars) or presence of 1 nm estrogen (light gray bars) (n = 3 each set). A, blocking Rho activity by cotransfection with C3 transferase was unable to prevent estrogen-induced activation of hSlo1 by DBDM-hERα. B, blocking PI3K activity with 25 μm LY294002 reduced estrogen-induced activation of hSlo1 by DBDM-hERα. C, scheme of hSlo1 transcriptional regulation. Estrogen via ERα and PI3K stimulate hSlo1 transcription. c-Src and ERK inhibit hSlo1 basal transcription. Dashed lines depict possible intermediates in the signaling cascade. ?, whether ERK is downstream of c-Src in this system is unknown. *, significantly different (p < 0.05).