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. 2011 Jul 1;286(36):31565–31575. doi: 10.1074/jbc.M111.227546

FIGURE 1.

FIGURE 1.

Deletion of whole N terminus (aa 1–63) of Orai3 disrupts its activation. a, amino acid sequence of Orai3 ΔN-term deletion mutant. Gray letters represent amino acids within the N terminus of Orai3 that were deleted. b, time course of whole cell inward currents at −74 mV activated by passive store depletion of HEK 293 cells coexpressing CFP-STIM1 and YFP-Orai3 ΔN-term in comparison with wild-type YFP-Orai3 (p < 0.01). c, current/voltage relationship corresponding to b. d, time course of whole cell currents at −74 and +74 mV of HEK 293 cells coexpressing CFP-STIM1 and YFP-Orai3 ΔN-term upon perfusion with 75 μm 2-APB (p < 0.01). Confocal fluorescence images from a representative cell expressing wild-type YFP-Orai3 (e) and YFP-Orai3 ΔN-term (f) are shown. Bars in fluorescence images correspond to 5 μm. pF, picofarad. Error bars represent S.E.