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. 2011 Jul 1;286(36):31565–31575. doi: 10.1074/jbc.M111.227546

FIGURE 3.

FIGURE 3.

Interaction of Orai3 N terminus with CaM. a, amino acid sequence of two Orai3 N-terminal peptides, aa 47–65 and aa 52–65. b, PAGE shift assay demonstrating Ca2+ dependence of CaM interaction with peptides aa 47–65 and aa 52–65 in 2 mm EGTA and 2 mm Ca2+ media at different peptide/CaM ratios. c, BIAcore sensorgrams displaying binding of CaM to peptide aa 47–65 compared with that of aa 52–65 in the presence of 2 mm Ca2+. On the right side, inactivation profiles of Orai3 ΔN1–46 and Orai3 ΔN1–51 are shown in accordance with the respective sensorgrams. RU, resonance units.