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. 2011 Jul 18;286(36):31241–31249. doi: 10.1074/jbc.M111.256024

FIGURE 6.

FIGURE 6.

ACh-evoked currents in neurons transfected with α4-eGFPβ4wt and α4-eGFPβ4-R348C nAChRs. The transfected neurons were identified with green fluorescence (A and D). ACh (300 μm) was puffed (0.1 s) onto voltage-clamped neurons (VH = −65 mV) and evoked inward currents (B and E). C, summary of 300 μm ACh-evoked currents (IACh) from neurons with different transfections (WT, α4-eGFPβ4wt; R348C, α4-eGFPβ4-R348C). F, summary of the density of 300 μm ACh-induced currents (IACh divided by membrane capacitance). Numbers of recorded neurons are indicated in parentheses.