TABLE 3.
IC50 values for phage-displayed TIMP-2 variants from the screening of libraries that mutate three regions on TIMP-2
The data were obtained by competitive phage ELISA as described under “Experimental Procedures.” All mutants contained WT sequences in L2 and L3.
| N-TIMP-2 variant | L1 sequence | IC50 |
Selectivity, MMP3/MMP1 | |
|---|---|---|---|---|
| MMP1cd | MMP3cd | |||
| nm | ||||
| WT | CSCSPV | 13 ± 4 | 13 ± 3 | 1.0 |
| TM1 | CVCQGL | 9 ± 3 | 54 ± 17 | 6.4 |
| TM8 | CDCAPV | 16 ± 3 | 140 ± 74 | 8.6 |
| TM10 | CVCKTS | 31 ± 9 | 96 ± 36 | 2.3 |
| TM13 | CSCVTE | 16 ± 4 | 36 ± 9 | 2.3 |
| TM14 | CSCNST | 15 ± 3 | 61 ± 13 | 4.0 |