FIGURE 5.

Effects of IMP-3 knockdown on IGFII gene expression during apoptosis. After 15-Gy IR, cells were immediately transfected with control siRNA, IMP-3 siRNA, or buffer only and maintained for 0–3 days. Cells were assessed for IGFII mRNA by qRT-PCR (A), intracellular protein by Western blot analysis (B), and secreted protein by ELISA of cell culture supernatants (C). The experiment was repeated three times independently. For each experiment, n = 6. Basal, cells not transfected and not exposed to IR. **, p < 0.01.