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. 2011 Jul 8;286(36):31684–31696. doi: 10.1074/jbc.M110.202531

FIGURE 5.

FIGURE 5.

TAIII-induced autophagy and cellular calcium mobilization. A, HeLa cells were treated with 10 μm TAIII and the cytosolic calcium levels were analyzed with calcium probe Fluo-4. Left panel, fluorescence microscopic image of Fluo-4-loaded cells treated with 10 μm TAIII or DMSO vehicle (control) for 30 min. Right panel, time course of changes of relative Fluo-4 fluorescence (TAIII/control) of TAIII-treated cells. B, HeLa cells expressing mRFP-LC3 were treated with 10 μm TAIII or DMSO vehicle (control) for 18 h. Cells were then loaded with Fluo-4 and examined with fluorescence microscope. C, HeLa cells expressing tfLC3 were treated with 20 μm BAPTA-AM or left untreated for 1 h, followed by treatment with 10 μm TAIII for 6 h. Scale bars, 20 μm. D, HeLa cells were treated as in C and LC3-II, p-S6K (Thr-389), and ULK1 expression was determined by immunoblot.