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. 2011 Jul 15;286(36):31347–31360. doi: 10.1074/jbc.M111.232413

FIGURE 1.

FIGURE 1.

TLR2, NOD1, and NOD2 cooperatively regulate maturation of human DCs. A–F, immature DCs (0.5 × 106 cells/ml) were cultured with GM-CSF and IL-4 and left untreated (Medium) or treated with TLR2 ligand Rv0754 (200 ng/ml) or NOD1 ligand C12-iE-DAP (1 μg/ml) or NOD2 ligand MDP (1 μg/ml) as well as with combinations of TLR2, NOD1, and NOD2 ligands for 48 h followed by analysis of the surface expression of maturation markers CD83 (A), CD40 (B), HLA-DR (C), CD80 (D), CD1a (E), and CD86 (F) by flow cytometry. Data are presented as mean fluorescence intensities (MFI) ± S.E. from six independent donors.