FIGURE 11.

PI3K and PKCδ signaling axis integrate with MAPK pathway during TLR2-, NOD1-, and NOD2-triggered maturation of human DCs. A and B, Rv0754, C12-iE-DAP, and MDP induce activation of ERK1/2 and p38 MAPK during CTLA-4-triggered (A) or TGF-β-triggered (B) immunosuppression of DCs. C and D, inhibition of MAPK activation upon NOTCH1 signaling inactivation using NOTCH1 siRNA as assayed by Western blot under CTLA-4-mediated (C) and TGF-β-mediated (D) suppression of human DCs. E–H, inhibition of PI3K by LY294002 (E and F) or PKCδ (G and H) by PKCδ inhibitor Rottlerin abrogates Rv0754-, C12-iE-DAP-, and MDP-triggered activation of ERK1/2 or p38 MAPK during CTLA-4-triggered (E, G, and H) or TGF-β-triggered (F) immunosuppression of DCs. The immunoblots are representative of three independent experiments.