Skip to main content
. 2011 Jul 15;286(36):31347–31360. doi: 10.1074/jbc.M111.232413

FIGURE 3.

FIGURE 3.

TLR2, NOD1, and NOD2 synergistically surmount CTLA-4- and TGF-β-mediated suppressed maturation of human DCs. A–C, DCs were pretreated with CTLA-4 (1 μg/ml) for 6 h followed by treatment with Rv0754 or C12-iE-DAP or MDP as well as with a combination of Rv0754, C12-iE-DAP, and MDP for an additional 42 h, and expression of maturation markers CD80 and CD86 (A), CD83 and HLA-DR (B), and CD40 and CD1a (C) was analyzed. D–F, Rv0754, C12-iE-DAP, and MDP induced synergistic maturation of human DCs under TGF-β-triggered immunosuppressive conditions as analyzed by surface expression of maturation markers CD80 and CD86 (D), CD83 and HLA-DR (E), and CD40 and CD1a (F). Data are presented as mean fluorescence intensities (MFI) ± S.E. from six independent donors. **, p < 0.05 versus CTLA-4 or TGF-β.