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. 2011 Jul 22;286(37):32300–32312. doi: 10.1074/jbc.M111.247866

FIGURE 1.

FIGURE 1.

DN HNF1α provokes an atypical ER stress response. A, map of HNF1α domain structure with positions of DN HNF1α frameshift mutations indicated by arrows. a, dimerization domain; b, DNA binding (POU-like) domain; c, homeodomain-like region. The gray box indicates the region mistranslated in the DN mutant due to the frameshifts. B, Western blot for HNF1α (4 μg of nuclear extracts/lane) after 0, 24, or 48 h of 500 ng/ml Dox. C, immunofluorescence for (DN) HNF1α after 48 h of Dox. Two non-expressing cells are marked with arrows. D, Q-PCR of ER stress genes with or without 48 h of Dox in INS DN Hnf1α cells (n = 4). E, Q-PCR of ER stress genes with or without 48 h of Dox in INS DN Hnf4α cells (n = 4). Error bars, S.E.