FIGURE 2.
ER stress-induced gene transcription is attenuated and cell death increased after induction of DN Hnf1α. A–D, Q-PCR of BiP, CHOP, protein-disulfide isomerase (PDI), and DnaJ C3 mRNA after a dose response of CPA with or without 24-h preinduction of DN Hnf1α (n = 3). E, MTT assay of similarly treated cells (n = 5). Absorbance (570 nm) of the samples was normalized to that of the control. F, cell death ELISAPLUS apoptosis assay of similarly treated cells (n = 4). Data are shown as absolute absorbance values. Error bars, S.E.