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. 2011 Jul 22;286(37):32300–32312. doi: 10.1074/jbc.M111.247866

FIGURE 3.

FIGURE 3.

DN HNF1α increases basal and stimulus-induced cytoplasmic calcium. A–C, DN Hnf1α cells expressing cytosolic aequorin with or without 24 h of 500 ng/ml Dox. A, calcium mobilization from the ER induced by the muscarinic agonist carbachol (100 μm) (n = 4). B, ER calcium uptake blocked by CPA (20 μm) (n = 3). C, plasma membrane depolarized by KCl (30 mm) (n = 3). D–F, DN Hnf1α expressing mitochondrially targeted aequorin with or without 24 h of 500 ng/ml Dox. Carbachol, CPA, or KCl was added as indicated (n = 3 for each experiment). G, DN Hnf1α expressing cytosolic aequorin with or without 24 h of 500 ng/ml Dox, basal calcium before and after removal of extracellular calcium by supplementation of EGTA (0.4 mm) (n = 7). H, DN Hnf1α expressing cytosolic aequorin with or without 24 h of 500 ng/ml Dox. Removal of extracellular calcium was as in G. Readdition of calcium following CPA was as indicated to monitor store-operated calcium influx (n = 4).