FIGURE 3.

Insulin secretion profiles in perifused islets. Islets isolated from age-matched (20–30-week-old), C3H/He (blue; n = 4 for A and n = 9 for B), SynKO (red; n = 3 for A and n = 8 for B), GrnKO (green; n = 4 for A and n = 8 for B), and DKO (purple; n = 3 for A and n = 8 for B) mice were perifused with standard 2.8 mm glucose-containing Krebs-Ringer buffer for 30 min. Thereafter, the collection of each fraction (1 ml/min) was started, and an appropriate secretagogue was applied at 10 min after the collection. For glucose stimulation (A), islets were perifused with the buffer containing 16.7 mm glucose for 30 min followed by the standard 2.8 mm glucose buffer for 20 min. For stimulation by high K⁺ concentration (B), islets were perifused with buffer containing 60 mm KCl plus 65 mm NaCl for 15 min followed by the standard buffer for 10 min.