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. 2011 Jul 18;286(37):32244–32250. doi: 10.1074/jbc.M111.268631

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Interaction between granuphilin-a with syntaxins 1–4 and Munc18-1–3 in MIN6 cells. A, MIN6 cells were infected with recombinant adenovirus encoding either LacZ or HA-tagged granuphilin-a (HA-Grn-a). The cell lysates were immunoprecipitated (IP) with anti-HA antibody, and the immune complexes along with 1:2000 volume of the original lysates (input) were immunoblotted with antibodies toward syntaxins 1–4 and HA. B, HEK293 cells were transfected with a plasmid encoding HA-Grn-a without (−) or with a plasmid encoding FLAG-Munc18-1–3. Proteins bound to anti-FLAG affinity gels were immunoblotted with antibodies toward HA and FLAG. Input corresponds to 1:200 volume of the original lysates.