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. 2011 Jul 22;286(37):32324–32332. doi: 10.1074/jbc.M111.254417

FIGURE 2.

FIGURE 2.

Effect of HFD feeding on Atg7 in hypothalamic arcuate nucleus. Male C57BL/6 mice were maintained on a normal chow diet versus an HFD, and brain sections were then prepared for Atg7 immunostaining (red). Data show Atg7 immunostaining across the hypothalamic arcuate nucleus (ARC) of chow-fed versus HFD-fed C57BL/6 mice. DAPI staining (blue) reveals the nucleus of all cells in the sections. At least five mice per group were analyzed. Data represent approximately 4–5 months of HFD versus chow feeding. 3V, third ventricle. Scale bar = 100 μm.