FIGURE 2.

Sterols and MVA-derived nonsterol(s) act synergistically to promote HMGal degradation. A, UT-2/HMGal cells were incubated for 20 h in Medium B supplemented with 50 μm lovastatin. Cells were pulse-labeled and chased in Medium B in the absence or presence of sterols (2 μg/ml 25-hydroxycholesterol + 20 μg/ml of cholesterol) with the indicated concentrations of MVA. HMGal was immunoprecipitated with anti-HMGR membrane region antibodies. B, quantification of the results in A. ■, no MVA; ●, 0.5 mm MVA; ▴, 2 mm MVA; ⧫, 20 mm MVA. C, cells were set up for the experiment and labeled as in A. Cells were chased in the presence of the indicated additions, which were added to the chase medium. The remaining HMGal at the end of the chase (%) is indicated.