FIGURE 6.

MVA-derived nonsterol product(s) accelerate degradation of HMGal in UT-2 cells. UT-2/HMGal cells were depleted of sterols and MVA by 20 h incubation in Medium B containing 50 μm lovastatin. The cells were pulse-labeled and chased for 6 h in Medium B supplemented without (lane 2) or with 20 mm MVA (lane 3) plus the indicated inhibitors: 250 μm ZOL (lanes 4), 150 μm ZA (lane 5), 10 μm NB-598 (lane 6), 5 μm RO-48–8071 (lane 7), 10 μm SKF-104976 (lane 8). HMGal was immunoprecipitated with anti-HMGR membrane region polyclonal antibodies. Densitometric analysis (bars) represents HMGal remaining at the end of the chase relative to cells chased without MVA (lane 2). Results are the mean ± S.E. of 4 independent experiments.