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. 2011 Jul 20;286(37):32606–32616. doi: 10.1074/jbc.M111.220574

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Dimerization analysis of the W89E substitution. Analytical size exclusion chromatography and ultracentrifugation was utilized to determine the oligomeric state of the W89E substitution. The calculated molecular mass of a ChxR_FL monomer or dimer is 25.8 or 51.6 kDa, respectively. A, wild-type ChxR_FL (solid line) eluted from an analytical size exclusion column as a dimer with an approximate molecular mass of 45 kDa, whereas ChxR_FL^W89E (dashed line) eluted from the column as a compact monomer with an approximate molecular mass of 16 kDa. B, wild-type ChxR_Rec (solid line) and ChxR_Rec^W89E (dashed line) eluted from an analytical size exclusion column as a dimer (21 kDa) and monomer (9.8 kDa), respectively. C, wild-type ChxR_FL (solid line) was determined to exist as a dimer through analytical ultracentrifugation with an approximate molecular mass of 46.6 kDa, whereas ChxR_FL^W89E (dashed line) was determined to exist as a monomer with an approximate molecular mass of 23.9 kDa.