FIGURE 5.

Comparison of rLytN with other murein hydrolases. A, an overnight culture of S. aureus Newman was diluted into fresh media supplemented with buffer, lysostaphin, mutanolysin, or recombinant LytN (rLytN), and growth was monitored over 12 h at 37 °C by A₆₀₀ readings. Conditions were performed in triplicate, and error bars represent the means ± S.E. B, staphylococci grown to stationary phase were washed in buffer and subsequently incubated in the presence of buffer alone, lysostaphin, mutanolysin, or rLytN. Cell lysis was monitored by A₆₀₀ over time as in A. C, purified staphylococcal peptidoglycan was incubated with buffer alone, lysostaphin, mutanolysin, or rLytN, and hydrolysis was measured by monitoring A₆₀₀ over 12 h.