FIGURE 3.

Immunolocalization of HDAC1, HDAC2, and HDAC3 in newborn kidneys. A–F, consecutive sections stained with HDAC1 or HDAC2 and Pax2 reveal overlapping expression patterns in nephrogenic zone (3,3′-diaminobenzidine immunoperoxidase method). G–I, consecutive sections stained with HDAC3 and WT1 reveal overlapping expression in glomerular podocytes (DAB immunoperoxidase method). J–M, immunofluorescent labeling on consecutive sections for HDAC1 and AQP2 or HDAC2 and Na,K-ATPase, showing enrichment of HDAC1 and HDAC2 in less differentiated nephron structures. PTA, pretubular aggregate; RV, renal vesicle; SB, S-shaped body; G, glomerulus; NZ, nephrogenic zone; CD, collecting duct; PT, proximal tubule.