FIGURE 7.

Direct actions of HDAC inhibition on mK4 gene expression. A, analysis of gene expression. Metanephric mesenchyme mK4 cells were cultured in the presence of Scriptaid or control drug Nullscript for 6 h followed by RNA extraction and RT-PCR. The majority of the gene expression changes is similar to those observed metanephric organ cultures treated with HDACi (compare with Fig. 4D). B, treatment of mK4 cells with Scriptaid (S), but not Nullscript (N) for 6 h stimulates H3 and H4 hyperacetylation and H3K4 trimethylation. C, ChIP assays. Chromatin immunoprecipitation was performed in mK4 cells using antibodies to acetyl-H3, acetyl-H3K9, and acetyl-H4 following treatment with Scriptaid or Nullscript. PCR density was factored to input, and results were expressed as fold change in relative enrichment of histone marks in Scriptaid/Nullscript-treated samples.