TABLE 4.
Cathepsin K and V cleavage sites in non-mutated recombinant fibrillin-1 fragments identified by N-terminal sequencing
The details for the fibrillin-1 sequence and numbering are identical to the legend for Table III.
| Fragment | Cleavage site |
Band number | Position in FBN1 | Domain | |
|---|---|---|---|---|---|
| P4–P1 | P1′–P12′ | ||||
| Cathepsin K | |||||
| rFBN1-N | CGSR | SIQHCNIRCMNG | 6 | 115 | Linker EGF1-EGF2 |
| EPPR | VLPVNVTDYCQL | 14 | 444 | End of proline-rich | |
| PPRV | LPVNVTD | 12 | 445 | End of proline-rich | |
| PGMT | SAGSDINECALD | 4 | 719 | Linker TB2-cbEGF7 | |
| rFBN1-C | GNCT | DVNECLDP | 5 | 1487 | N-terminus of fragment |
| Cathepsin V | |||||
| rFBN1-N | GSRS | IQHCNIRCMNGG | 7 | 116 | Linker EGF1-EGF2 |
| PGMT | SAGSDINECALD | 2 | 719 | Linker TB2-cbEGF7 | |
| rFBN1-C | GNCT_ | DVNECLDP | 1 | 1487 | N-terminus of fragment |
| CPYG | SGIIVGPDDSAV | 8, 9, 10, 11 | 2115 | Linker TB6-cbEGF32 | |