FIGURE 3.

CoCl₂-induced hypoxia regulates AU-rich-containing mRNAs. A, shown is the relative quantity of ARE mRNAs measured by RT-qPCR comparing normoxia versus hypoxia after 36 h of CoCl₂ treatment, and β-actin serves as a loading control. B, the relative amounts of ARE mRNAs expressed in UM74B cells transfected with HuR siRNA or control siRNA after CoCl₂ treatment were measured by RT-qPCR. The values are normalized using a factor calculated from β-actin gene expression. C and D, the decay rates of the c-Myc mRNA and β-actin as indicated were assessed in UM74B cells by RT-qPCR after transcription inhibition with actinomycin D. Error bars denote the S.E. of three sets of experiments. *, p < 0.05; n = 3.