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Amino acid specificity of CapD, CP, and F24H. The specific activity for each enzyme, CapD (gray), CP (light gray), and F24H (dark gray), was determined using 5 mm amino acid acceptor as described under “Experimental Procedures.” The specific activity for l-Tyrosine (Tyr*) was assayed at 1.8 mm amino acid due to the limited solubility of tyrosine. Each activity was measured in triplicate, and S.D. values are given in supplemental Table S2.
