FIGURE 9.
Uncompetitive inhibition of RPE65 isomerohydrolase by PBN in the liposome-based isomerohydrolase assay. Lineweaver-Burk plots of 11-cis-retinol generated by RPE65. Liposomes with increasing concentrations of all-trans-retinyl palmitate (S) were incubated with equal amounts (125 μg) of chicken RPE65 expressed in 293A cells, with (■) and without (○) PBN (0.1 mm).