FIGURE 2.

The effects of replacement mutations and slyA on the β-galactosidase produced by a FimB-LacZ fusion. A, effects of mutations rm21 and rm22 in the presence of a wild type (dark bars) and deleted (light bars) slyA gene. Error bars indicate 95% confidence values calculated from four replicate experiments. Strains BGEC905, KCEC1243, KCEC1077, KCEC1271, KCEC1079, and KCEC1273, listed in supplemental material [strains-pdf], were grown in rich-defined glycerol medium and processed as described under “Experimental Procedures.” B, effect of 0.1 mm IPTG (light bars) versus 0 mm IPTG (dark bars) in wild type, ΔslyA and ΔslyA lacUV5-slyA backgrounds. Strains BGEC905, KCEC1334, KCEC1494, and KCEC1765 were used. Error bars and experimental conditions as A.