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. 2011 Jul 15;286(37):32026–32035. doi: 10.1074/jbc.M111.266619

FIGURE 6.

FIGURE 6.

Western blot analysis of slyA expression. 68.5 ng of purified SlyA (P; lane 1), or 62 μg of total protein isolated from the strains indicated (lanes 2–8), were subject to SDS-PAGE and Western blot analysis as described under “Experimental Procedures.” Extracts were made from the wild type strain (BGEC905) grown in rich-defined glycerol medium (wt R; lane 2) or minimal glycerol medium (wt M; lane 3). All the additional strains analyzed (lanes 4–8) were grown in rich-defined glycerol medium. All extracts were prepared from cultures grown to an A600 = 0.2. Lane 4 (slyA) ΔslyA mutant (KCEC1334); lanes 5 and 6, the lacUV5-slyAUUG construct (KCEC1494); lanes 7 and 8, the lacUV5-slyAAUG construct (KCEC1765). The lac fusion constructs were grown in the absence (−I) or presence (+I) of 1 mm IPTG as indicated.