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. 2011 Jul 20;286(37):32628–32637. doi: 10.1074/jbc.M111.244632

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — URD domain binds substrates FANCD2 and FANCI. A, structural representation of the surface hydrophobic patch mutants of the URD domain. B, pulldown of substrates His-FANCD2 and FLAG-FANCI with WT URD and the patch mutants of the URD, with the mutations indicated in A. URD input and URD incubated with beads alone control are shown using anti-FANCL antibody. The pulldown shows either His-FANCD2 with anti-His or FLAG-FANCI with anti-FLAG and the URD species that have been pulled down with anti-FANCL antibody.