TABLE 2.
Identification of differentially expressed proteins between the pcDNA3.1 A HEK293 cells and pcDNA3.1 A-CUL4B HEK293 cells
| Spot no. | Protein description | Mr/pI | Matched peptides | Sequencing coverage (100%) | NCBI accession No. | Mascot score |
|---|---|---|---|---|---|---|
| A. Up-regulated proteins in pcDNA3.1A-CUL4B HEK293 cells | ||||||
| 7 | F-actin capping protein α-1 subunit | 32966/5.45 | 8/19 | 49 | 5453597 | 167 |
| 9 | Eukaryotic translation initiation factor 3, subunit 2β, 36 kDa | 36607/5.38 | 9/19 | 54 | 4503513 | 268 |
| B. Down-regulated proteins in pcDNA3.1A-CUL4B HEK293 cells | ||||||
| 1 | Homebox prox1 | 28099/6.19 | 8/18 | 38 | 7706322 | 183 |
| 2 | Chaperonin containing TCP1, subunit 6A isoform a | 58212/6.23 | 3/9 | 30 | 4502643 | 87 |
| 3 | RuvB-like 1 | 50404/6.02 | 9/21 | 29 | 4506753 | 288 |
| 5 | Proteasome α1 subunit isoform | 29665/6.15 | 3/11 | 35 | 14506179 | 72 |
| 10 | Ras-GTPase-activating protein SH3-domain-binding protein | 52308/5.36 | 7/31 | 19 | 5031703 | 152 |
| 11 | Endoplasmic reticulum protein 29 isoform 1 precursor | 29071/6.77 | 3/11 | 39 | 5803013 | 81 |
| 13 | Peroxiredoxin III | 25854/6.00 | 6/25 | 21 | 32483377 | 67 |