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. 2011 Jul 22;286(37):32790–32800. doi: 10.1074/jbc.M111.245985

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Adiponectin suppresses DOX-induced apoptosis in heart. A, representative photographs of heart sections stained with TUNEL from WT and APN-KO mice at 5 days after DOX or vehicle injection. Apoptotic nuclei were determined by TUNEL staining (green). Myocytes were determined by sarcomeric actinin (red), and total nuclei were counterstained with DAPI (blue). High magnification representative photographs of heart sections are shown in the bottom panels. Scales bars, 40 μm (top) and 10 μm (bottom). B, quantitative analysis of apoptotic nuclei from WT (n = 5) and APN-KO mouse (n = 5) hearts following DOX or vehicle injection. TUNEL-positive nuclei were counted in several randomly selected fields and expressed as a percentage of the total number of nuclei. C, detection of cleaved caspase-3 from WT (n = 5) and APN-KO mouse (n = 5) hearts following DOX or vehicle injection by Western blot analysis. D, quantitative analysis of apoptotic nuclei from WT (n = 5) and APN-KO (n = 5) mouse hearts treated with Ad-APN or Ad-β-gal following DOX injection. TUNEL-positive nuclei were counted in several randomly selected fields and expressed as a percentage of the total number of nuclei. E, detection of cleaved caspase-3 from WT (n = 5) and APN-KO (n = 5) mouse hearts treated with Ad-APN or Ad-β-gal following DOX injection by Western blot analysis. F, effect of DOX on cultured myocytes. Representative photographs of TUNEL-positive cardiac myocytes under conditions of serum deprivation with or without DOX stimulation for 24 h in the presence or absence of adiponectin protein are shown. Apoptotic nuclei were identified by TUNEL staining (green), and total nuclei were identified by DAPI counterstaining (blue). G, quantitative analysis of TUNEL-positive cells with DOX or vehicle exposure in the presence or absence of adiponectin. TUNEL-positive nuclei were counted in several randomly selected fields and expressed as a percentage of the total number of nuclei (n = 4). H, detection of cleaved caspase-3 with DOX or vehicle exposure in the presence or absence of adiponectin.

FIGURE 2. — Adiponectin suppresses DOX-induced apoptosis in heart. A, representative photographs of heart sections stained with TUNEL from WT and APN-KO mice at 5 days after DOX or vehicle injection. Apoptotic nuclei were determined by TUNEL staining (green). Myocytes were determined by sarcomeric actinin (red), and total nuclei were counterstained with DAPI (blue). High magnification representative photographs of heart sections are shown in the bottom panels. Scales bars, 40 μm (top) and 10 μm (bottom). B, quantitative analysis of apoptotic nuclei from WT (n = 5) and APN-KO mouse (n = 5) hearts following DOX or vehicle injection. TUNEL-positive nuclei were counted in several randomly selected fields and expressed as a percentage of the total number of nuclei. C, detection of cleaved caspase-3 from WT (n = 5) and APN-KO mouse (n = 5) hearts following DOX or vehicle injection by Western blot analysis. D, quantitative analysis of apoptotic nuclei from WT (n = 5) and APN-KO (n = 5) mouse hearts treated with Ad-APN or Ad-β-gal following DOX injection. TUNEL-positive nuclei were counted in several randomly selected fields and expressed as a percentage of the total number of nuclei. E, detection of cleaved caspase-3 from WT (n = 5) and APN-KO (n = 5) mouse hearts treated with Ad-APN or Ad-β-gal following DOX injection by Western blot analysis. F, effect of DOX on cultured myocytes. Representative photographs of TUNEL-positive cardiac myocytes under conditions of serum deprivation with or without DOX stimulation for 24 h in the presence or absence of adiponectin protein are shown. Apoptotic nuclei were identified by TUNEL staining (green), and total nuclei were identified by DAPI counterstaining (blue). G, quantitative analysis of TUNEL-positive cells with DOX or vehicle exposure in the presence or absence of adiponectin. TUNEL-positive nuclei were counted in several randomly selected fields and expressed as a percentage of the total number of nuclei (n = 4). H, detection of cleaved caspase-3 with DOX or vehicle exposure in the presence or absence of adiponectin.