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. 2011 Jun 6;208(6):1243–1252. doi: 10.1084/jem.20102477

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© 2011 Schwickert et al.

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Figure 1. — GC occupancy is regulated by competition. (A) Diagrammatic representation of the experimental protocol. (B) Histology showing composition of GCs in draining lymph nodes 6 d after immunization. B1-8^lo B cells are GFP⁺ (false-colored red), and B1-8^hi B cells are CD45.1⁺ (green). GCs were identified by absence of IgD⁺ B cells (blue) within the B cell follicle. Images are representative of multiple GCs from two independent experiments. (C) Composition of GCs by flow cytometry. Gated on CD19⁺Igλ⁺FAS⁺GL-7⁺. (left) Representative flow cytometry plots showing the fraction of cells of each genotype (B1-8^lo B cells are CFP⁺, B1-8^hi B cells are CD45.1⁺, and endogenous B cells are CFP⁻CD45.1⁻). (right) Quantification of GC occupancy under different conditions. Each symbol represents one mouse. Data are from two independent experiments with two to three mice per condition per experiment.

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