Figure 5.
Access to T cell help drives early B cell competition. (A) Diagrammatic representation of the experimental protocol. (B) Flow cytometric analysis of transferred B1-8hiDEC205+/+CFP+ and B1-8hiDEC205−/−CD45.1+ CFSE-labeled B cells in draining lymph nodes 4 d after immunization. Gated on CD19+Igλ+CD45.1+ (B1-8hiDEC205+/+) or CD19+Igλ+CFP+ (B1-8hiDEC205+/+) cells. (top) Representative CFSE dilution profile. (bottom) Representative FAS/GL-7 plot. (C) Quantification of data in B. Each symbol represents one mouse. Data are from three independent experiments with one to two mice per condition per experiment. CS, P. falciparum circumsporozoite protein (used as a control antigen).