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. 2011 Jun 6;208(6):1253–1265. doi: 10.1084/jem.20101751

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© 2011 Goncalves et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — The in vitro killing of L. major by GR1⁺ monocytes from wild-type mice. (A) GR1⁺ (left) and GR1⁻ (right) monocytes were sorted from CX3CR1^GFP/+ RAG2^−/− mice by GFP expression and incubated with 10:1 ratio of metacyclic RFP-L. major in vitro. Fluorescence photomicrographs of gently washed monolayers were taken after 1 h (top) or 3 h (bottom). (B) The total mean number of L. major parasites (n = 3) associated with GR1⁺ or GR1⁻ monocytes after 1 or 3 h in vitro infection. (C) GR1⁺ and GR1⁻ monocytes were sorted based on CX3CR1^GFP expression and incubated with 5:1 ratio of L. major to monocytes in vitro. The cells were kept in culture for 3 d at 37°C without washing. After 3 d, parasite numbers were calculated by serial dilution assays. Data represent the mean ± SE of 3 independent experiments using a total of 40 mice. Bars, 50 μm.