Figure 2.

Hydrothermal pretreatment at 180°C using 0.5% w/w solids concentration. Glucose and xylose yields from co-hydrolysis compared with those from separate pretreatment and enzymatic hydrolysis (SPEH) for pretreatment of 0.5% w/w Populus slurries in water alone at 180°C followed by enzymatic hydrolysis over a range of enzyme loadings from 15 + 5 to 105 + 35 mg of cellulase plus xylanase, respectively, per gram of glucan and xylan in the raw biomass. The eight stacked bars on the left show pretreatment at log R₀ of 3.6 and those on the right at log R₀ of 4.0. The hatched left bar of each immediately adjacent pair shows the distribution of glucose and xylose recovery from pretreatment and enzymatic hydrolysis for the conventional SPEH approach, and the solid bar on the right of each pair presents the amounts of glucose and xylose released from the overall co-hydrolysis method. The error bars represent the standard errors, based on three replicates.