Table 1.
Chimeric primers for RT and primers for PCR.
| Primers | Sequences |
| For RT | |
| Rat VHL | |
| Short | 5'-CGGCTCGTGACTGCTATCTTCAATGTGATGTTGGCAAAAAT-3' |
| Long | 5'-CGGCTCGTGACTGCTATCTTC(N)nAATGTGATGTTGGCAAAAAT-3' |
| Rat Collagen I | |
| Short | 5'-CGGCTCGTGACTGCTATCTTCCCTTGGCACCATCGTTGC-3' |
| Long | 5'-CGGCTCGTGACTGCTATCTTC(N)nCCTTGGCACCATCGTTGC-3' |
| For PCR | |
| Rat VHL | |
| Upper | 5'-GGGACCCATGATGGACTTCT-3' |
| Lower | 5'-CGGCTCGTGACTGCTATCTTC-3' |
| Rat Collagen I | |
| Upper | 5'-CCCTGGTGAACGTGGTGTAC-3' |
| Lower | The same as the VHL lower primer |
Gene-specific sequences: letters in italic; a universal sequence: bold letters; the linkers (N)n: either (A)10, (C)10, (G)10, (AT)5, or (A)5(G)5(A)5.