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Primers and hydrolysis probes used in real-time PCR.
| Gene | TaqMan probe No | Forward primer 5'→3' | Reverse primer 5'→3' |
|---|---|---|---|
| CGB | #71 Roche Diagnostic, Cat. No: 04688945001 |
TACTGCCCCACCATGACC | CACGGCGTAGGAGACCAC |
| GNRH1 | #29 Roche Diagnostic, Cat. No: 04687612001 |
GACCTGAAAGGAGCTCTGGA | CTTCTGGCCCAATGGATTTA |
| HPRT | Human HPRT Gene Assay (Roche Diagnostic, Cat. No: 05046157001) | ||
