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. 2011 Sep 14;6(9):e24141. doi: 10.1371/journal.pone.0024141

Figure 1. Ara-LAM mediated changes in MAPK signaling cascade in Leishmania donovani–infected macrophages.

Figure 1

Macrophages were pretreated with Ara-LAM for 3 hr or in some cases followed by Leishmania infection for 15, 30, 60, and 120 min. The cells were then lysed and subjected to Western blot with anti-pp38MAPK, p38MAPK and pERK1/2, ERK1/2, as described in Materials and Methods (A). In separate experimental sets, cells were transfected with control siRNA or TLR2-specific siRNA for 24 hr washed and then treated with Ara-LAM followed by L. donovani for 30 min. Western blot analysis was performed to analyze the expression of anti-pp38MAPK, p38MAPK and pERK1/2, ERK1/2 (B).