(A) Hippocampal neurons were fixed shortly after plating and immunolabeled with a neuron-specific anti- tubulin antibody (red), an anti Sec8 antibody (green) and a nuclear marker (blue). The right panel shows a pseudocolor image of the Sec8 only. Scale bar 5 . (B) Experimental results, Sec8, (open circles) compared with numerical simulations, , (red line) for the round neuron shown in the inset. Initial profile (random perturbations) in black. In this case , , , , , and . (C) Temporal evolution of the simulation shown in (B) ( axis, membrane position; axis, time; color scale, normalized values). Results are normalized to the final maximum value of .